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  • Title: Evaluation of decontamination methods and growth media for primary isolation of Mycobacterium ulcerans from surgical specimens.
    Author: Yeboah-Manu D, Bodmer T, Mensah-Quainoo E, Owusu S, Ofori-Adjei D, Pluschke G.
    Journal: J Clin Microbiol; 2004 Dec; 42(12):5875-6. PubMed ID: 15583329.
    Abstract:
    We evaluated four decontamination methods and one nondecontamination procedure in combination with four egg-based media for the primary isolation of Mycobacterium ulcerans from tissue specimens. With mycobacterial recovery and contamination rates of 75.6 and 2.4%, respectively, the combination of the oxalic acid decontamination method with Lowenstein-Jensen medium supplemented with glycerol yielded the best results.
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