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  • Title: Comparison of five methods for extraction of Legionella pneumophila from respiratory specimens.
    Author: Wilson D, Yen-Lieberman B, Reischl U, Warshawsky I, Procop GW.
    Journal: J Clin Microbiol; 2004 Dec; 42(12):5913-6. PubMed ID: 15583339.
    Abstract:
    The efficiencies of five commercially available nucleic acid extraction methods were evaluated for the recovery of a standardized inoculum of Legionella pneumophila in respiratory specimens (sputum and bronchoalveolar lavage [BAL] specimens). The concentrations of Legionella DNA recovered from sputa with the automated MagNA Pure (526,200 CFU/ml) and NucliSens (171,800 CFU/ml) extractors were greater than those recovered with the manual methods (i.e., Roche High Pure kit [133,900 CFU/ml], QIAamp DNA Mini kit [46,380 CFU/ml], and ViralXpress kit [13,635 CFU/ml]). The rank order was the same for extracts from BAL specimens, except that for this specimen type the QIAamp DNA Mini kit recovered more than the Roche High Pure kit.
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