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  • Title: Development and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus types 1 to 4.
    Author: Ito M, Takasaki T, Yamada K, Nerome R, Tajima S, Kurane I.
    Journal: J Clin Microbiol; 2004 Dec; 42(12):5935-7. PubMed ID: 15583346.
    Abstract:
    The fluorogenic TaqMan reverse transcriptase PCR (RT-PCR) assay was developed for detecting each of the dengue virus (DV) types 1 to 4. DV genome was detected in all the 35 serum samples from confirmed dengue cases by the TaqMan RT-PCR, although it was not detected in 13 and 21% by conventional type-specific and cross-reactive RT-PCR, respectively.
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