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  • Title: [Expression of OsBP-73 gene requires involvement of its intron in rice].
    Author: Chen J, Wang ZY.
    Journal: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao; 2004 Feb; 30(1):81-6. PubMed ID: 15583413.
    Abstract:
    It has been demonstrated in our previous work that rice OsBP-73 gene contains two exons interrupted by a 2471 bp intron. Here it was reported that the 5' flanking region of the ATG translation start codon in the first exon of OsBP-73 gene (from -1818 to +215) can not direct GUS gene expression in resistant rice calli or transgenic rice. When the complete OsBP-73 intron and its flanking region (from -1818 to +2844) are constructed in frame with GUS coding region, GUS activity can be detected in the resistant rice calli and transgenic rice. Experimental data also show that the complete OsBP-73 intron itself has no promoter activity. These results suggest that the OsBP-73 intron sequence is involved in directing the GUS gene expression. Another experiment demonstrates that the complete intron sequence can enhance the activity of OsEBP-89 gene promoter.
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