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  • Title: [Random amplified polymorphic DNA analysis of the genomes among 7 species of ticks].
    Author: Yang YS, Zhao HB, Di Wu JX, Zhang JJ, Shi ZY.
    Journal: Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi; 2004 Aug; 22(4):223-6. PubMed ID: 15587156.
    Abstract:
    OBJECTIVE: To study genomic polymorphic DNA and genetic distance of 7 species of ticks. METHODS: Ticks used in this study were Dermacentor nuttalli, D. silvarum, Haemaphysalis qinghaiensis, H. formosensis, H. punctata, Amblyomma testudinarium, and Ixodes ovatus. DNA extracts of the 7 species of ticks were amplified by random amplified polymorphic DNA (RAPD) and PCR technique using 5 primers with different arbitrary single chain polynucleotide sequences. DNA fingerprint maps were analyzed and the genetic distance among 7 species of ticks were counted. RESULTS: The amplified products of the 7 species of ticks by RAPD all showed their specific DNA band. The average genetic distance among them was 0.71. CONCLUSION: RAPD can differentiate the 7 species of ticks.
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