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  • Title: Bypassing the effect of zona pellucida changes on embryo formation following cryopreservation of bovine oocytes.
    Author: Mavrides A, Morroll D.
    Journal: Eur J Obstet Gynecol Reprod Biol; 2005 Jan 10; 118(1):66-70. PubMed ID: 15596275.
    Abstract:
    OBJECTIVE: To evaluate the effects of zona pellucida changes following the cryopreservation of bovine oocytes using two different techniques. DESIGN: Prospective randomised study. SETTING: University research facility and IVF research laboratory, NURTURE, University of Nottingham, UK. INTERVENTION: 508 bovine oocytes were collected from abattoir-derived ovaries and cryopreserved by slow freezing using standard straws and a new vitrification method using our self-constructed cryoloops. After thawing, the oocytes were inseminated by ICSI and standard IVF. MAIN OUTCOME MEASURES: Oocyte degeneration and oocyte cleavage. RESULTS: Cleavage rates after IVF in fresh oocytes was higher (49.5%) than the cryopreservation groups (slow freezing: 15.4% and vitrification: 25.8%), whereas after ICSI, the rates were similar (fresh: 17.3%, slow freezing: 9.4%, vitrification: 16.0%). CONCLUSIONS: Zona pellucida changes after oocyte cryopreservation significantly reduce the embryo formation rates after IVF. ICSI bypasses this complication and in selected species it could be an appropriate method of insemination.
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