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  • Title: Ectoine accumulation in Brevibacterium epidermis.
    Author: Onraedt A, De Muynck C, Walcarius B, Soetaert W, Vandamme E.
    Journal: Biotechnol Lett; 2004 Oct; 26(19):1481-5. PubMed ID: 15604783.
    Abstract:
    As a halotolerant bacterial species, Brevibacterium epidermis DSM 20659 can grow at relatively high salinity, tolerating up to 2 M NaCl. It synthesizes ectoine and the intracellular content increases with the medium salinity, with a maximum of 0.14 g ectoine/g CDW at 1 M NaCl. Sugar-stressed cells do not synthesize ectoine. Ectoine synthesis is also affected by the presence of external osmolytes. Added betaine is taken up and completely replaced ectoine, while L-proline is only temporarily accumulated after which ectoine is synthesized. The strain can metabolize ectoine; L-glutamate is a better carbon source for ectoine synthesis than L-aspartate.
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