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  • Title: Detection and characterization of rabies virus in Southern Brazil by PCR amplification and sequencing of the nucleoprotein gene.
    Author: Bordignon J, Brasil-Dos-Anjos G, Bueno CR, Salvatiera-Oporto J, Dávila AM, Grisard EC, Zanetti CR.
    Journal: Arch Virol; 2005 Apr; 150(4):695-708. PubMed ID: 15614435.
    Abstract:
    Due to the medical and socio-economical importance of both human and animal rabies infection, several studies have suggested the use of molecular techniques such as RT-PCR and DNA sequencing for diagnosis and phylogenetic studies of the rabies virus. Considering the conservancy of the nucleoprotein (N) gene of the virus, we herein describe a RT-PCR assay for rabies diagnosis and characterization. A total of 75 samples obtained from a variety of animal species in the state of Santa Catarina (SC), Southern Brazil, were comparatively studied by fluorescence antibody test (FAT), mouse inoculation test (MIT), cell infection assay and RT-PCR, which revealed itself to be as sensitive as FAT and MIT and less time-consuming than MIT. Direct sequencing of the 5' end of the N gene allowed the clustering of the SC samples with samples from the vampire bat-related or sylvatic cycle through comparative sequence analysis.
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