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  • Title: [Polymorphism analysis of the GOLA-DRB3 gene digested with HaeIII in Mongolian goat and Kazakh goat].
    Author: Sun DX, Zhang Y, Li N.
    Journal: Yi Chuan; 2004 Jan; 26(1):55-8. PubMed ID: 15626668.
    Abstract:
    The exon2 of GOLA-DRB3 gene was amplified and a uniform fragment of 285 bp was obtained in Mongolian Goat and Kazakh Goat. The 285bp PCR product was digested with restriction endomuclease HaeIII and genetic polymorphism was investigated by PCR-RFLP. Seventeen kinds of genotypes were found in two populations,which were controlled by seven alleles. There are significant differences in some genotypic frequencies and gene frequencies between the two populations (P<0.10, P<0.05, P<0.01); The results of chi(2) test showed that genotypes of GOLA-DRB3 gene in two populations did not fit with Hardy-Weinberg equilibrium (P<0.01).
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