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  • Title: [Preparation and characterization of monoclonal antibody against ARL-1 protein].
    Author: Shan J, Zhang JQ, Ma D, Bao HX, Miao FQ, Xie W.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2005 Jan; 21(1):60-3. PubMed ID: 15629086.
    Abstract:
    AIM: To prepare monoclonal antibodies against aldose reductase-like(ARL-1)protein. METHODS: Purified ARL-1 protein (ARL-GST) was used to immunize BALB/c mice, and mAbs were prepared by hybridoma technique. The mAbs against ARL-1 were detected by ELISA, Western blot and immunohistochemical staining respectively. RESULTS: One clone of hybridoma secreting specific mAb against ARL-1 was obtained. The Ig subclass of mAb was of IgG2b and the titre of the antibody in ascites was 1:4.096 x 10(5). Highexpression of ARL-1 protein in liver cancer was detected. CONCLUSION: The specific mAb against ARL-1 lays the foundation for investigation of functions of ARL-1 protein and relationship between ARL-1 protein and liver cancer.
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