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  • Title: [Determination of protopine and isocorydine in root of Dactylicapnos scandens by HPLC].
    Author: Yan TQ, Yang YF, Ai TM.
    Journal: Zhongguo Zhong Yao Za Zhi; 2004 Oct; 29(10):961-3. PubMed ID: 15631083.
    Abstract:
    OBJECTIVE: To establish a HPLC method for determination of protopine and isocorydine in root of Dactylicapnos scandens. METHOD: The separation was performed in a PHENOMENEX-C18 column with a mobile phase of methanol-0.2% phosphoric acid (adjusted to pH 7.0 with triethylamine)(50:50), The detection wavelength was at 254 nm and the flow rate was 0.8 mL x min(-1). RESULT: The average recovery of Protopine and Isocorydine was 97.9%, 98.6% respectively, and RSD 1.3%, 1.4%. CONCLUSION: This method is accurate, simple and reliable. It can be used for quality control of D. scandens.
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