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  • Title: CpG methylation of the CENP-B box reduces human CENP-B binding.
    Author: Tanaka Y, Kurumizaka H, Yokoyama S.
    Journal: FEBS J; 2005 Jan; 272(1):282-9. PubMed ID: 15634350.
    Abstract:
    In eukaryotes, CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B (CENP-B) specifically binds to the centromeric 17 base-pair CENP-B box DNA, which contains two CpG dinucleotides. In this study, we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs, and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA. Competition analyses revealed that the affinity of CENP-B for the CENP-B box DNA is reduced nearly to the level of nonspecific DNA binding by CpG methylation.
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