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  • Title: The temperature-sensitive ion channel TRPV2 is endogenously expressed and functional in the primary sensory cell line F-11.
    Author: Bender FL, Mederos Y Schnitzler M, Li Y, Ji A, Weihe E, Gudermann T, Schäfer MK.
    Journal: Cell Physiol Biochem; 2005; 15(1-4):183-94. PubMed ID: 15665528.
    Abstract:
    In sensory neurons heat is transduced by a subfamily of TRP channels sharing sequence homology with the capsaicin-sensitive vanilloid receptor subtype 1 (TRPV1), but differing in their thermal response thresholds. To identify a neuronal cell line endogenously expressing noxious heat-transducing ion channels, we examined F-11 cells, a hybridoma derived from rat dorsal root ganglia and mouse neuroblastoma. Using RT-PCR, transcripts homologous to TRPV2 and TRPV4, but not to TRPV1 or TRPV3, were found. We isolated a full-length cDNA of 2.4 kb coding for a 757-amino acid protein corresponding to mouse TRPV2, which was further characterized by immunocytochemistry and electrophysiology. Using the whole-cell patch-clamp technique, we observed a heat-evoked increase in outward and inward currents with a threshold of 51.6 +/- 0.2 degrees C. The current-voltage relationship stimulated by a temperature of 52 degrees C was characterized by an outward rectification with a reversal potential close to -10 mV. Heat-evoked currents could be inhibited by ruthenium red. There was no activation by stimulation with capsaicin or 2-aminoethoxydiphenyl borate. Our results indicate that F-11 cells express functional noxious heat-sensitive TRPV2 channels. Thus, we propose that F-11 cells represent a valuable in vitro model to characterize the properties of TRPV2 in a native neuronal environment.
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