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  • Title: The CD19 molecule is crucial for MID-dependent activation of tonsillar B cells from children.
    Author: Hadzic R, Forsgren A, Cardell LO, Riesbeck K, Wingren AG.
    Journal: Scand J Immunol; 2005 Feb; 61(2):165-72. PubMed ID: 15683453.
    Abstract:
    The Moraxella immunoglobulin (Ig) D-binding protein (MID) induces a strong proliferative response in human peripheral blood IgD+ B cells from adults isolated by positive selection using anti-CD19-conjugated microbeads. Here, we show that tonsillar B cells from children isolated with positive selection are unable to respond to MID stimulation. The proliferative response was very low or absent at various concentrations of MID tested and at different time points analysed, whereas the MID response of tonsillar B cells from adults isolated with positive selection was considerably higher. Tonsillar B cells from children isolated with positive selection responded to formalin-fixed preparations of Moraxella catarrhalis and Staphylococcus aureus Cowan strain I. In comparison to cells isolated with positive selection, a much higher proliferative response was recorded in tonsillar B cells from children isolated with negative selection, indicating that occupation of the CD19 molecule (i.e. positive selection) inhibited the response. Indeed, the addition of anti-CD19 monoclonal antibodies (MoAb) to MID-activated tonsillar B cells from children isolated with negative selection strongly inhibited the proliferative response. In contrast, anti-CD21 MoAb at the same concentration did only show a minor inhibition on the MID-induced response. Pre-incubation of tonsillar B cells isolated from children with anti-CD19 or anti-CD21 MoAb did not affect the binding of biotin-conjugated MID as analysed by flow cytometry. These results suggest that MID-activated tonsillar B cells from children have a strong requirement for signalling through the CD19 molecule. Future experiments will further reveal the importance of CD19 and possibly other molecules for optimal activation of tonsillar B cells isolated from both children and adults.
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