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  • Title: [Determination of scopoletin and umbelliferone contents in Saussurea medusa Maxim by high-performance liquid chromatography].
    Author: Su WW, Zhao J, Lin JM.
    Journal: Di Yi Jun Yi Da Xue Xue Bao; 2005 Jan; 25(1):119-20. PubMed ID: 15684018.
    Abstract:
    The contents of scopoletin and umbelliferone in Saussurea medusa Maxim were determined by high-performance liquid chromatography (HPLC) using Merck Lichrospher100 RP-18e column (250 mm x 4.0 mm, 5 microm) with the mobile phase of methanol-tetrahydrofuran-water solution and the absorbance of the compounds observed at 346 nm. HPLC yielded an average recovery and RSD of 100.07% and 1.42% for scopoletin, and 99.41% and 2.06% for umbelliferone, respectively, suggesting that HPLC is a simple, rapid and accurate method for quality control of Saussurea medusa Maxim.
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