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  • Title: Simple determination of riluzole in rat brain by high-performance liquid chromatography and spectrophotometric detection.
    Author: Maltese A, Maugeri F, Drago F, Bucolo C.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2005 Mar 25; 817(2):331-4. PubMed ID: 15687003.
    Abstract:
    A simple method was developed for separation and quantification of riluzole in rat brain. The analyses were performed by high-performance liquid chromatography using a C18 reversed-phase column (Hypersil ODS) with UV detection at 264 nm. The mobile phase consisted of methanol-water containing 1% triethylamine adjusted with orthophosphoric acid to pH 3.2. The retention time was 8.6 min. A simple liquid-liquid extraction with ethyl acetate was used to obtain riluzole from brain samples. The limit of quantification was 10 ng/g. The recovery was about 80%. The relationship between peak areas and concentrations was linear over the range between 0.01 and 0.8 microg/g, with r2 value over 0.99. The assay provided good reproducibility and accuracy and proved to be suitable for pharmacokinetic studies of riluzole.
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