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  • Title: Immobilization of single-stranded DNA by self-assembled polymer on gold substrate for a DNA chip.
    Author: Taira S, Yokoyama K.
    Journal: Biotechnol Bioeng; 2005 Mar 30; 89(7):835-8. PubMed ID: 15690340.
    Abstract:
    We developed a self-assembling polymer based on polyallylamine (PAH) for use in DNA chips. Thioctic acid (TA) was covalently attached to PAH in sidechains to immobilize the polymer on a gold surface by self-assembly. N-hydroxysuccinimide-ester terminated probe single-stranded (ss) DNA is easily covalently immobilized onto a TA-PAH-coated gold surface. Finally, the surface was covered with polyacrylic acid, which formed ion complexes with the TA-PAH, to reduce the cationic charge. This ssDNA on a polymer-coated surface recognized a fully matched DNA sequence and restrained nonspecific adsorption of target DNA. The selectivity and efficiency of hybridization was affected by adjusting the ionic strength of sodium chloride.
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