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  • Title: Template DNA ratio can affect detection by genus-specific PCR-denaturing gradient gel electrophoresis of bacteria present at low abundance in mixed populations.
    Author: Zhang L, Danon S, Grehan M, Lee A, Mitchell H.
    Journal: Helicobacter; 2005 Feb; 10(1):80-2. PubMed ID: 15691318.
    Abstract:
    The detection of Helicobacter species by genus-specific polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was compared with that by species-specific PCR in murine intestinal samples. Results suggest that, in samples containing multiple Helicobacter species, genus-specific PCR-DGGE may fail to detect all Helicobacter species present and that this relates to the initial template DNA ratio.
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