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  • Title: Simultaneous and selective production of levan and poly(gamma-glutamic acid) by Bacillus subtilis.
    Author: Shih IL, Yu YT.
    Journal: Biotechnol Lett; 2005 Jan; 27(2):103-6. PubMed ID: 15703872.
    Abstract:
    Bacillus subtilis(natto) Takahashi, used to prepare the fermented soybean product natto, was grown in a basal medium containing 5% (w/w) sucrose and 1.5% (w/w) L-glutamate and produced 58% (w/w) poly(gamma-glutamic acid) and 42% (w/w) levan simultaneously. After 21 h, 40-50 mg levan ml-1 had been produced in medium containing 20% (w/w) sucrose but without L-glutamate. In medium containing L-glutamic acid but without sucrose, mainly poly(gamma-glutamic acid) was produced.
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