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  • Title: [Study on the effect of leptin on fibroblast proliferation and collagen synthesis in vitro in rats].
    Author: Li P, Jin H, Liu D, Gao L, Xu Z, Nan W, Wang Y, Gao L.
    Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi; 2005 Jan; 19(1):20-2. PubMed ID: 15704835.
    Abstract:
    OBJECTIVE: To investigate the effect of leptin on fibroblast proliferation and collagen synthesis as to elucidate that fibroblasts play a role in leptin's effect on wound healing. METHODS: Purified dermal fibroblasts were derived from sucking wistar rat skin and exposed to leptin at concentration of 0, 10, 50, 100, 200, and 400 ng/ml. The survived fibroblasts were assessed by the colorimetric thiazolyl blue (MTT) assay. Replication of fibroblast was quantified by the incorporation of 3H-thymidine. Collagen synthesis of fibroblast cell was measured by the incorporation of 3H-proline into collagenase-sensitive protein. RESULTS: The absorption of fibroblast exposed to leptin at concentration of 200 and 400 ng/ml 0.082 +/- 0.013, 0.091 +/- 0.018 was higher than that of control group 0.063 +/- 0.010, P < 0.05. The incorporations of 3H-thymidine of fibroblast exposed to leptin at concentration of 200 and 400 ng/ml 379 +/- 101 cpm, 326 +/- 33 cpm were significantly higher than those of control group 219 +/- 56 cpm, P < 0.05. The incorporations of 3H-proline of fibroblast exposed to leptin at concentration of 200 and 400 ng/ml 911 +/- 55 cpm, 1 072 +/- 259 cpm were significantly higher than that of control group 679 +/- 176 cpm, P < 0.05. CONCLUSION: Leptin can promote rat cutaneous fibroblast proliferation and collagen synthesis in vitro. This suggests that cutaneous fibroblast plays a role in leptin's promoting skin wound healing and it may be one of the main mechanisms by which leptin enhances skin wound healing.
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