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  • Title: [Construction of fast propagation system of Houttuynia cordata new line].
    Author: Wu W, Zheng YL, Liu F, Tan G, Ren HY, Zhang WC.
    Journal: Zhongguo Zhong Yao Za Zhi; 2004 Jan; 29(1):24-8. PubMed ID: 15709373.
    Abstract:
    OBJECTIVE: To construct the fast propagation system of Houttuynia cordata Thunb. new line by selecting the proper type and concentration of plant growth regulators and proper explants. METHOD: The different explants of Houttuynia cordata new line were cultured on MS media with different concentration of different plant grow regulators. RESULT: Stem node over-ground and shoot tip were optimum explants for fast propagation. The stem which node grew on MS medium with KT at 0.5 mg x L(-1) could induce more leaves and grow higher. The shoot tip cultured on MS medium with 1.0 mg x L(-1) 6-BA could induce better callus. Adventitious shoots were better achieved from over-ground nodal explants cultured on MS medium supplemented with 6-BA at 1.0 mg x L(-1) and NAA at 0.2 mg x L(-1), or 6-BA at 0.5 mg x L(-1) and NAA at 0.2 mg x L(-1). MS medium with IAA at 0.2 mg x L(-1) was the best one on inducing roots. CONCLUSION: The propagat coefficient can be highly improved by inducing adventitious shoots through stem node over-ground, and thus plentiful seeds for production can be provided.
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