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  • Title: Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28-9.
    Author: Huang CJ, Wang TK, Chung SC, Chen CY.
    Journal: J Biochem Mol Biol; 2005 Jan 31; 38(1):82-8. PubMed ID: 15715951.
    Abstract:
    Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in Taiwan. This bacterium exhibited biocontrol potential on Botrytis leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. At least two chitinases (ChiCW and ChiCH) were excreted by B. cereus 28-9. The ChiCW-encoding gene was cloned and moderately expressed in Escherichia coli DH5alpha. Near homogenous ChiCW was obtained from the periplasmic fraction of E. coli cells harboring chiCW by a purification procedure. An in vitro assay showed that the purified ChiCW had inhibitory activity on conidial germination of Botrytis elliptica, a major fungal pathogen of lily leaf blight.
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