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  • Title: Mutational analysis of the adcCBA genes in Streptococcus gordonii biofilm formation.
    Author: Mitrakul K, Loo CY, Gyurko C, Hughes CV, Ganeshkumar N.
    Journal: Oral Microbiol Immunol; 2005 Apr; 20(2):122-7. PubMed ID: 15720574.
    Abstract:
    Streptococcus gordonii, a primary colonizer, is part of the pioneer biofilm consortium that initiates dental plaque development on tooth surfaces. An insertion of Tn917-lac transposon into the adcR gene produced a biofilm-defective phenotype. S. gordonii adcR is a regulatory gene and is part of an operon (adc) that includes three other genes, adcCBA. AdcC contains a putative consensus-binding site for adenosine triphosphate, AdcB is a putative hydrophobic membrane protein, and AdcA is a putative lipoprotein permease. Mutants were constructed by insertional inactivation in each of the three adcCBA genes and their effects on biofilm formation examined. The adcC::spec(R) and adcB::spec(R) mutations displayed a biofilm-defective phenotype, whereas the adcA::spec(R) mutant was biofilm-positive in a static polystyrene microtiter plate biofilm assay. All three mutants formed poor biofilms in a flow-cell system and were competence-defective, suggesting the adc operon plays an important role in S. gordonii biofilm formation and competence.
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