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  • Title: Analysis of polyamine metabolism in soybean seedlings using 15N-labelled putrescine.
    Author: Ohe M, Kobayashi M, Niitsu M, Bagni N, Matsuzaki S.
    Journal: Phytochemistry; 2005 Mar; 66(5):523-8. PubMed ID: 15721944.
    Abstract:
    The translocation and metabolism of polyamines during soybean germination were studied using 15N-labelled putrescine as a precursor. Both 15N-labelled and unlabelled polyamines were simultaneously detected using a novel application of ionspray ionization-mass spectrometry. 15N-putrescine was rapidly transported to the shoots and roots, where it was converted to spermidine and spermine. The main 15N-polyamine that accumulated in the root was 15N-spermine. It was found that there were differences in the way endogenous putrescine and exogenous 15N-putrescine were metabolized in soybean seedlings.
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