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  • Title: Purification and on-column refolding of EGFP overexpressed as inclusion bodies in Escherichia coli with expanded bed anion exchange chromatography.
    Author: Cabanne C, Noubhani AM, Hocquellet A, Dole F, Dieryck W, Santarelli X.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2005 Apr 15; 818(1):23-7. PubMed ID: 15722040.
    Abstract:
    The enhanced green fluorescent protein (EGFP) was over-expressed in Escherichia coli as inclusion bodies to increase its quantity and to facilitate its purification. Insoluble EGFP has been purified on Q Hyper Z matrix by expanded bed adsorption after solubilization in 8 M urea. The adsorption was made in expanded bed mode to avoid centrifugation. EBA-column refolding was done by elimination of urea and elution with NaCl. The EGFP was obtained as a highly purified soluble form with similar behavior in fluorescence and electrophoresis as native EGFP.
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