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  • Title: Protein identification using 2D-LC-MS/MS.
    Author: Delahunty C, Yates JR.
    Journal: Methods; 2005 Mar; 35(3):248-55. PubMed ID: 15722221.
    Abstract:
    Multidimensional liquid chromatography techniques have been coupled to tandem mass spectrometry to provide a robust method to identify proteins in complex mixtures. Data acquisition is interfaced directly with search algorithms for identification through cross-correlation with databases. This review describes the most recent advances in methodologies for protein identification by mass spectrometry and describes the limitations of the application of the technologies.
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