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  • Title: An integral approach towards a practical application for a plant-made monoclonal antibody in vaccine purification.
    Author: Pujol M, Ramírez NI, Ayala M, Gavilondo JV, Valdés R, Rodríguez M, Brito J, Padilla S, Gómez L, Reyes B, Peral R, Pérez M, Marcelo JL, Milá L, Sánchez RF, Páez R, Cremata JA, Enríquez G, Mendoza O, Ortega M, Borroto C.
    Journal: Vaccine; 2005 Mar 07; 23(15):1833-7. PubMed ID: 15734051.
    Abstract:
    The use of transgenic plants for the production of pharmaceutical compounds has received increasing attention in the last few years. However, many technological and regulatory issues regarding the practical exploitation of this alternative system of production remain to be solved; a situation that explains the lack of commercial products derived from such a system. This paper reports the expression in transgenic plants and cells of a single-chain antibody variable-region fragment (scFv) and a mouse monoclonal antibody to the hepatitis B virus surface antigen (HBsAg). The large-scale purification of the scFv from plants and its use for immunopurification of HBsAg are also described, together with elements concerning regulatory issues and technologies for compliance with good manufacturing and agricultural practices.
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