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  • Title: A chromism-based assay (CHROBA) technique for in situ detection of protein kinase activity.
    Author: Tomizaki KY, Jie X, Mihara H.
    Journal: Bioorg Med Chem Lett; 2005 Mar 15; 15(6):1731-5. PubMed ID: 15745830.
    Abstract:
    A unique chromism-based assay technique (CHROBA) using photochromic spiropyran-containing peptides has been firstly established for detection of protein kinase A-catalyzed phosphorylation. The alternative method has advantages that avoid isolation and/or immobilization of kinase substrates to remove excess reagents including nonreactive isotope-labeled ATP or fluorescently-labeled anti-phosphoamino acid antibodies from the reaction mixture. Such a novel protocol based on thermocoloration of the spiropyran moiety in the peptide can offer not only an efficient screening method of potent kinase substrates but also a versatile analytical tool for monitoring other post-translational modification activities.
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