These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Analysis of plasma amino acids by HPLC with photodiode array and fluorescence detection.
    Author: Schwarz EL, Roberts WL, Pasquali M.
    Journal: Clin Chim Acta; 2005 Apr; 354(1-2):83-90. PubMed ID: 15748603.
    Abstract:
    BACKGROUND: Plasma amino acids are usually analyzed by ion-exchange chromatography (IEC), a reproducible but time consuming method. Here, we test whether plasma amino acids can be analyzed using reverse-phase high performance liquid chromatography (HPLC). METHODS: Filtered plasma, with S-carboxymethyl-l-cysteine as the internal standard, was derivatized and analyzed by an Agilent 1100 HPLC system. Primary amino acids were derivatized with o-phthalaldehyde 3-mercaptopropionic acid (OPA) and detected by a diode array detector. Secondary amino acids were derivatized with 9-fluorenylmethyl chloroformate (FMOC) and detected fluorometrically. Chromatographic separation is achieved by two gradient elutions (two injections per sample), starting at different pHs, on a reverse phase Agilent Zorbax Eclipse C(18) column AAA (4.6 x 150 mm). RESULTS: The HPLC method evaluated correlated well with IEC (0.89</=r</=1.00) with linearity up to 2500 mumol/l. The between- and within-run CVs were <6.0%. In addition, this method is able to separate argininosuccinic acid, homocystine and allo-isoleucine, rare but clinically significant amino acids. CONCLUSION: This HPLC method was comparable to IEC and could represent an alternative for amino acid analysis.
    [Abstract] [Full Text] [Related] [New Search]