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  • Title: Application of phage-displayed single chain antibodies in western blot.
    Author: Ding YL, Liu MY, Han W, Yang SL, Liu H, Gong Y.
    Journal: Acta Biochim Biophys Sin (Shanghai); 2005 Mar; 37(3):205-9. PubMed ID: 15756424.
    Abstract:
    A phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens.
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