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  • Title: Molecular profiling of Bacteroides spp. in human feces by PCR-temperature gradient gel electrophoresis.
    Author: Pang X, Ding D, Wei G, Zhang M, Wang L, Zhao L.
    Journal: J Microbiol Methods; 2005 Jun; 61(3):413-7. PubMed ID: 15767017.
    Abstract:
    A group-specific PCR-based temperature gradient gel electrophoresis (TGGE) method was developed to study the population composition of genus Bacteroides in human gut. Highly reproducible and well-separated bands in TGGE fingerprints of ten unrelated human fecal samples showed complex and host-specific Bacteroides species composition. Dynamic monitoring over 22 months of samples from one healthy 10-year-old boy indicated a relatively stable population profile of Bacteroides. The species identity of each band in TGGE gel of this boy was also resolved via comigration analysis of sequenced inserts in a Bacteroides group-specific clone library. This work provides a rapid and effective technique for analyzing the species composition of Bacteroides in human gut.
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