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  • Title: Inserting foreign peptides into the major coat protein of bacteriophage M13.
    Author: Ilyichev AA, Minenkova OO, Kishchenko GP, Tat'kov SI, Karpishev NN, Eroshkin AM, Ofitzerov VI, Akimenko ZA, Petrenko VA, Sandakhchiev LS.
    Journal: FEBS Lett; 1992 Apr 27; 301(3):322-4. PubMed ID: 1577174.
    Abstract:
    Foreign DNA fragments were inserted into filamentous phage gene VIII to create hybrid B-proteins with foreign sequences in the amino terminus. The hybrid proteins are incorporated into the virions which retain viability and infectivity. Virions with hybrid B-proteins have the same contour length and the same number of B-protein molecules as virions with natural B-proteins. It was shown that for one of hybrid B-proteins the position of the processing site had changed.
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