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  • Title: [Temperature-dependent functional changes in sarcoplasmic reticulum membranes].
    Author: Klebanov GI, Poglazov AF, Nikushkin EV, Shubin MV, Rubtsov BV.
    Journal: Biofizika; 1979; 24(4):699-703. PubMed ID: 157782.
    Abstract:
    Temperature effect on changes in calcium ion transport and activity of Ca-ATPase of sarcoplasmic reticulum vesicles (SRV) was studied. Several temperature intervals of the change of SRV functional activity parameters were found: 8-12 degrees C-beginning of accumulation of SRV Ca2+, 12-19 degrees sharp activation of Ca2+ accumulation, 27-32 degrees-activation of passive yield, 36-43 degrees-reversible change of the activity of the accumulation system and activation of Ca yield, 48 degrees and higher--irreversible denaturation of transport Ca-ATPase. Coincidence of temperature intervals of SRV structural reconstructions, detected by means of fluorescent probes and functional changes points to a close relationship between structural reconstructions and functional responses of biological membranes.
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