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  • Title: Fluorescence imaging of the activity of glucose oxidase using a hydrogen-peroxide-sensitive europium probe.
    Author: Wu M, Lin Z, Schäferling M, Dürkop A, Wolfbeis OS.
    Journal: Anal Biochem; 2005 May 01; 340(1):66-73. PubMed ID: 15802131.
    Abstract:
    A method for optical imaging of the activity of glucose oxidase (GOx) using a fluorescent europium(III) tetracycline probe for hydrogen peroxide is presented. A decay time in the microsecond range and the large Stokes shift of 210 nm of the probe facilitate intensity-based, time-resolved, and decay-time-based imaging of glucose oxidase. Four methods for imaging the activity of GOx were compared, and rapid lifetime determination imaging was found to be the best in giving a linear range from 0.32 to 2.7 m Unit/mL. The detection limit is 0.32 m Unit/mL (1.7 ng mL(-1)) which is similar to that of the time-resolved (gated) imaging using a microtiterplate reader. Fluorescent imaging of the activity of GOx is considered to be a useful tool for GOx-based immunoassays with potential for high-throughput screening, immobilization studies, and biosensor array technologies.
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