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  • Title: Efficiency of adenovirus-mediated gene transduction in heart grafts in rats.
    Author: Fujishiro J, Kawana H, Inoue S, Shimizu H, Yoshino H, Hakamata Y, Kaneko T, Murakami T, Hashizume K, Kobayashi E.
    Journal: Transplant Proc; 2005; 37(1):67-9. PubMed ID: 15808549.
    Abstract:
    AIM: We determined the characteristics of transgene expression of heart grafts following ex vivo gene transfer using an adenovirus vector. Transgene expression was assessed periodically in the same animals by a non-invasive bioimaging system. METHODS: Rat heterotopic heart transplantation was performed in a syngenic combination. We infused 1 x 10(9) plaque-forming units of adenovirus vectors containing firefly luciferase gene into the heart graft via the coronary artery, with preservation at 4 degrees C and transplanted into the cervix of the recipient. Transgene expression was periodically visualized and quantified by a noninvasive bioimaging system without sacrificing experimental animals. RESULTS: Transgene expression in the graft peaked at day 7 and then fell gradually. Transgene expression was also observed in the recipient liver. CONCLUSIONS: We have determined the time course of transgene expression in the heart graft. This constitutes important information about ex vivo gene therapy for heart grafts.
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