These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: New tool for metabolic pathway engineering in Escherichia coli: one-step method to modulate expression of chromosomal genes.
    Author: Meynial-Salles I, Cervin MA, Soucaille P.
    Journal: Appl Environ Microbiol; 2005 Apr; 71(4):2140-4. PubMed ID: 15812048.
    Abstract:
    A simple and highly efficient method was developed to produce a library of Escherichia coli clones that express a particular chromosomal gene at a wide range of expression levels. The basic strategy was to replace all or part of the upstream region of a coding sequence containing the elements involved in its expression (promoter, operator, gene coding for a regulator, ribosome binding site, and start codon) with a PCR-generated library of expression cassettes.
    [Abstract] [Full Text] [Related] [New Search]