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  • Title: Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis.
    Author: Easterday WR, Van Ert MN, Simonson TS, Wagner DM, Kenefic LJ, Allender CJ, Keim P.
    Journal: J Clin Microbiol; 2005 Apr; 43(4):1995-7. PubMed ID: 15815042.
    Abstract:
    A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.
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