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  • Title: [Determination of protein in urine by spectral probe of molybdenum(VI) salicylfluoronate complex].
    Author: Shen H, Liu L, Liu Y.
    Journal: Guang Pu Xue Yu Guang Pu Fen Xi; 1999 Jun; 19(3):444-6. PubMed ID: 15819086.
    Abstract:
    The absorption spectral behavior of binding reaction of molybdenum(VI)-salicylfluoronate on bovine serum albumin was investigated. A novel method for the determination of protein in urine using Mo(VI)-salicylfluoronate complex as a spectroprobe was developed. The maximum binding number (n) of molybdenum complex to BSA was found to be 100. The molar absorptivity of binding product at 575 nm was 2.86 x 10(6) L x mol(-1) x cm(-1). The method possesses high sensitivity as well as high selectivity. It could be used to determine protein in human urine directly. The relative standard deviation for the determination is 1.5%.
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