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  • Title: Difference in the response to PIF/activin between animal caps excised from mid- or late blastula stages of Xenopus laevis.
    Author: Brun R, Brun-Zinkernagel AM.
    Journal: Experientia; 1992 Apr 15; 48(4):405-8. PubMed ID: 1582499.
    Abstract:
    Animal caps from Xenopus embryos at Stage 7/8 cultured in salt solution, were capable of elongating, and formed various embryonic tissues including axial mesoderm, nervous tissues and pigmented retina. In contrast, animal caps from Stage 9 only developed into permanent blastulae. Following exposure to PIF/activin, however, such animal caps displayed morphogenetic movements. They formed tube-shaped embryoids that frequently had one or more tails but no head. We conclude that animal caps from Stage 9 produce more reliable results than those from Stage 7/8 because the latter are most likely contaminated with mesodermal cells.
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