These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Transamidinase activity of hyphal fractions of a streptomycin-producing Streptomyces griseus strain and enhancement of enzyme activity in a nonproducing mutant.
    Author: Barabás G, Szeszák F, Szabó G.
    Journal: Antimicrob Agents Chemother; 1974 Jul; 6(1):11-5. PubMed ID: 15828164.
    Abstract:
    The presence of transamidinase (TA-se) was demonstrated in Streptomyces griseus strain 45 which does not produce streptomycin (SM). This enzyme is involved in the biosynthesis of streptidine, a component of the SM molecule. TA-se is generally thought to be present only in SM-producing Streptomyces strains. The recent results are, however, in agreement with out previous data which showed the presence of streptidine in the isolated cell wall of SM-nonproducing strain 45. The TA-se activity of this strain is very low, but we were able to increase it by addition of arginine or by increasing the incubation with myo-inositol. The addition of these substances to the cultures of SM-nonproducing S. griseus strains, which hitherto were deficient in TA-se, may serve as a method for detecting the presence of this enzyme. In an incubation system in which the hyphae of an SM-producing strain of S. griseus had been blended and separated by density gradient centrifugation, certain hyphal fractions had been combined, and their SM production and TA-se activity had been measured, we were unable to find any close correlation between TA-se activity and SM-producing capacity. The hyphal fractions and their combination did not show strict parallelism between TA-se activity and the level of SM production.
    [Abstract] [Full Text] [Related] [New Search]