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  • Title: Isotachophoretic determination of carboxylic acids in biodegradation samples.
    Author: Zgoła-Grześkowiak A, Grześkowiak T, Zembrzuska J, Frańska M, Frański R, Lukaszewski Z.
    Journal: J Chromatogr A; 2005 Mar 18; 1068(2):327-33. PubMed ID: 15830939.
    Abstract:
    In the current study a method of isotachophoretic separation of selected carboxylic acids was developed. The method was used for the determination of carboxylated oligo(ethylene glycol)s and their degradation products in biodegradation tests of PEG 250 DA [a mixture of dicarboxylated oligo(ethylene glycol)s]. Two tests were performed in the studies: the Organization for Economic Cooperation and Development (OECD) screening test and the river water die-away test. Both the biodegradation tests proved relatively fast biodegradation of the studied compounds. In the OECD screening test the biodegradation was faster than in the river water die-away test which can be ascribed to a higher concentration of bacteria in the biodegradation liquor. The minimal sample pretreatment and relatively low cost of analysis by the isotachophoretic method used here make it a good alternative to existing methods of carboxylic acids analysis.
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