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  • Title: Detection of spores of Bacillus anthracis using the polymerase chain reaction.
    Author: Carl M, Hawkins R, Coulson N, Lowe J, Robertson DL, Nelson WM, Titball RW, Woody JN.
    Journal: J Infect Dis; 1992 Jun; 165(6):1145-8. PubMed ID: 1583336.
    Abstract:
    The polymerase chain reaction (PCR) was used to identify spores of Bacillus anthracis. By using an assay capable of amplifying a 1247-bp fragment from the gene that encodes the edema factor of B. anthracis, as few as 10(3) copies of a plasmid containing the edema factor gene and as few as 2 x 10(4) spores were detected. Subjecting the product of this PCR to a second PCR designed to amplify a 208-bp fragment nested within the 1247-bp product improved detection to a single plasmid copy per PCR and to two spores of B. anthracis per PCR.
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