These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Denaturation of metalloproteins with EDTA to facilitate enzymatic digestion and mass fingerprinting.
    Author: Janecki DJ, Reilly JP.
    Journal: Rapid Commun Mass Spectrom; 2005; 19(10):1268-72. PubMed ID: 15834845.
    Abstract:
    Metal ions bound to a protein often stabilize tertiary and/or quaternary structure. Consequently, the digestion of metalloproteins that precedes analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is frequently incomplete. It is demonstrated that ethylenediaminetetraacetic acid (EDTA) successfully destabilizes metalloprotein structure and thereby facilitates tryptic digestion and protein identification.
    [Abstract] [Full Text] [Related] [New Search]