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  • Title: Flow cytometry: a new method to investigate the properties of water-in-oil-in-water emulsions.
    Author: Hai M, Bernath K, Tawfik D, Magdassi S.
    Journal: Langmuir; 2004 Mar 16; 20(6):2081-5. PubMed ID: 15835655.
    Abstract:
    We present a new and facile method to evaluate w/o/w emulsions containing fluorescent markers by flow cytometry. Flow cytometry allows simultaneous measurement of w/o/w emulsion droplets "marked" with a fluorescent marker or "blank" without the need for complicated sample preparation. The yield of preparation of the w/o/w emulsion and the release rate of the fluorescent marker FITC-BSA were investigated by this new method. The release fraction (after 24 h) of FITC-BSA from the w/o/w emulsion decreased with increasing concentration of FITC-BSA inside the internal phase, just like the release fraction of NaCl as marker from the w/o/w emulsion. Flow cytometry results show that the yield and release behavior in w/o/w emulsions are in agreement with results reported by more complicated methods.
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