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  • Title: [High-performance liquid chromatography combined with mass spectrum analysis for chromatographic fingerprinting of compound dandelion enema].
    Author: Yan Y, Liu ST, Xu ZY, Luo QZ, Tan YF, Chen ZL.
    Journal: Di Yi Jun Yi Da Xue Xue Bao; 2005 Apr; 25(4):447-9, 453. PubMed ID: 15837653.
    Abstract:
    OBJECTIVE: To identify the active components of compound dandelion enema, a preparation from 7 traditional Chinese herbal drugs for treatment of gynecological diseases. METHODS: Three-dimensional high-performance liquid chromatography (3D-HPLC) was employed to separate the ethyl acetate extract of compound dandelion Enema, and HPLC combined with mass spectrum (MS) analysis used for chromatographic fingerprinting. RESULT: By comparing the ionic fragments of MS and retention time of each peak, the main active components in compound dandelion enema were determined, including caffeic acid, ferulic acid and protocatechualdehyde. CONCLUSION: HPLC coupled with mass spectroscopy can be used for qualitative analysis of compound dandelion enema.
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