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  • Title: [Population studies of Exophiala jeanselmeis by sequence analysis in the ribosomal DNA].
    Author: Li DM, Li RY, Wang XH, Wan Z, Ma SQ, Wang DL.
    Journal: Beijing Da Xue Xue Bao Yi Xue Ban; 2005 Apr 18; 37(2):167-71. PubMed ID: 15841147.
    Abstract:
    OBJECTIVE: To study the populations of Exophiala jeanselmei strains from diverse sources using rDNA sequences. METHODS: Tested strains included 20 isolates of Exophiala jeanselmei (type culture, reference culture, clinical and environmental isolates), 8 isolates of E. moniliae, 1 isolate of Exophiala werneckii, respectively. DNA was isolated with boiled-iced water method. Ribosomal DNA and its internal transcribed spacer regions were amplified and analyzed with sequencing. RESULTS: The ribosomal genes of E. jeanselmei varied greatly. Twenty strains of this species were divided into 7 types. The genetic distances of some strains in this species were quite long. CONCLUSION: The ribosomal RNA genes and their internal transcript sequences vary more widely than those of the other species in this genus.
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