These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [High performance liquid chromatogram (HPLC) determination of adenosine phosphates in rat myocardium].
    Author: Miao Y, Wang CL, Yin HJ, Shi DZ, Chen KJ.
    Journal: Beijing Da Xue Xue Bao Yi Xue Ban; 2005 Apr 18; 37(2):201-2. PubMed ID: 15841156.
    Abstract:
    OBJECTIVE: To establish method for the quantitative determination of adenosine phosphates in rat myocardium by optimized high performance liquid chromatogram (HPLC). METHODS: ODS HYPERSIL C(18) column and a mobile phase of 50 mmol/L tribasic potassium phosphate buffer solution (pH 6.5), with UV detector at 254 nm were used. RESULTS: The average recovery rates of myocardial adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) were 99%-107%, 96%-104% and 95%-119%, respectively; relative standard deviations (RSDs) of within-day and between-days were less than 1.5% and 5.1%, respectively. CONCLUSION: The method is simple, rapid and accurate, and can be used to analyse the adenosine phosphates in myocardium.
    [Abstract] [Full Text] [Related] [New Search]