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  • Title: Microarray-based genotyping for blood groups: comparison of gene array and 5'-nuclease assay techniques with human platelet antigen as a model.
    Author: Bugert P, McBride S, Smith G, Dugrillon A, Klüter H, Ouwehand WH, Metcalfe P.
    Journal: Transfusion; 2005 May; 45(5):654-9. PubMed ID: 15847651.
    Abstract:
    BACKGROUND: Most blood group alloantigens specific for red cells and platelets (PLTs) are based on single-nucleotide polymorphisms (SNPs) in genes encoding relevant membrane proteins. STUDY DESIGN AND METHODS: By use of five human PLT antigen (HPA) systems as a model, the suitability of a fourth-generation microarray technique for SNP typing was investigated. The results of the former were compared with those of a parallel developed third-generation technique (TaqMan assay, Applied Biosystems). Both techniques were validated by use of a unique panel of 71 blinded DNA samples containing at least 15 aa, bb, and ab genotypes for the HPA-1, -2, -3, -5, and-15 systems. RESULTS: Unambiguous and concordant results were obtained with both techniques for all samples. CONCLUSION: The data presented here validate the use of microarray for large-scale SNP typing for clinically relevant blood group alloantigens.
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