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  • Title: Contractility of rat gubernacula affected by calcitonin gene-related peptide and beta-agonist.
    Author: Tomiyama H, Hutson JM.
    Journal: J Pediatr Surg; 2005 Apr; 40(4):683-7. PubMed ID: 15852279.
    Abstract:
    PURPOSE: The effects of calcitonin gene-related peptide (CGRP), isoprenaline, and guanethidine on the neonatal rat gubernaculum were investigated in organ culture with the aim of seeing whether beta -agonists or beta -antagonists are involved in gubernacular development during testicular descent. METHODS: A total of 200 gubernacula were studied. The gubernacula (n = 20 for each experiment) from male rat pups were incubated in aerated culture medium with isoprenaline (1 and 10 micromol/L) with/without CGRP (714 nmol/L) or guanethidine (1 and 10 micromol/L) with/without CGRP (714 nmol/L). Cultures were observed for 5 days by video camera to see spontaneous rhythmic contractions, which are known to correlate with normal gubernacular migration in the rodent. RESULTS: Of gubernacula cultured without CGRP, 60% showed contractions by day 5, and the groups of isoprenaline and 10 micromol/L guanethidine showed similar rates, but only 15% of the 1 micromol/L guanethidine group showed contractility (P < .005). In contrast, of the gubernacula cultured with CGRP, each group showed high contractility from day 2 of the culture, and there was no difference between each group and the CGRP-alone group. CONCLUSIONS: Although the beta -blocker showed a weak inhibitory effect on de novo gubernacular contractility, CGRP had a stronger effect on contractility, overriding any possible effects of the beta -neuron pathway. The contractility of the rat gubernaculum during testicular descent is primarily mediated by the genitofemoral sensory nerve fibers via release of CGRP, but beta -adrenergic nerves also may be involved.
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