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  • Title: The rat spinal cord slice: Its use in generating pharmacological evidence for cholinergic transmission using the alpha7 subtype of nicotinic receptors in the central autonomic nucleus.
    Author: Bradaïa A, Seddik R, Schlichter R, Trouslard J.
    Journal: J Pharmacol Toxicol Methods; 2005; 51(3):243-52. PubMed ID: 15862469.
    Abstract:
    Lamina X surrounds the central canal of the spinal cord and is an important site for the convergence of somatic and visceral afferent inputs relaying nociceptive information. Lamina X contains sympathetic preganglionic neurons (SPN) in the so-called central autonomic nucleus which may participate to viscero-autonomic reflexes. Here, we describe a transversal slice preparation of postnatal rat thoracolumbar spinal cord which allows the detailed characterization of the morphology, electrophysiological properties, synaptic activities and receptor pharmacology of neurons surrounding the central canal. By means of the patch clamp technique, in its whole cell configuration, and by the use of various pharmacological tools, we show here that lamina X neurons of the central autonomic nucleus express functional alpha7 nicotinic receptors which are located postsynaptically on SPNs where they are involved in a fast cholinergic transmission. Thus, this in vitro preparation is useful to study the mechanisms and the pharmacology of viscero-autonomic reflexes.
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